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The existence of three independent rodent mutant cell lines (murine scid, V-3 and irs-20) The cells were fused by treatment with 2 ml of 47% PEG (in serum-free medium containing 10% dimethyl sulfoxide) for 1 min followed by washing in serum-free medium containing 10% dimethyl sulfoxide. After 24 h, the cells were trypsinized and split into three 10 cm dishes with selective medium

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Severe combined immune deficiency (SCID) is a fatal, heterogeneous group of immune disorder, characterized by T-cell lymphopenia, a profound lack of cellular (T-cell) and humoral (B-cell) immunity

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